Get a Quote
| Name | Monkeypox virus (MPXV) antibody | |||||||||
| Catalog Number | R386t7 | R387c6 | R392a3 | R396t5 | R395a6 | R399e2 | R401c8 | R400e7 | R417t6 | R418t3 |
| Immunogen | Recombinant MPXV A35R antigen | Recombinant MPXV A29L antigen | Recombinant MPXV B6R antigen | Recombinant MPXV M1R antigen | ||||||
| Category | Fully human monoclonal antibody | |||||||||
| Reactive Species | Monkeypox virus | |||||||||
| Application Platform & Recommended Pairs | Immunochromatography (ICA) | |||||||||
| R386t7 (Capture) - R392a3 (Detection) R387c6 (Capture) - R392a3 (Detection) |
R396t5 (Capture) - R399e2 (Detection) R396t5 (Capture) - R395a6 (Detection) |
R401c8 (Capture) - R400e7 (Detection) | R417t6 (Capture) - R418t3 (Detection) | |||||||
| Purity | Protein A/G purified, purity >95% | |||||||||
| Buffer | 1xPBS, pH 7.4 | |||||||||
| Storage | Store at -20°C or below, aliquot to avoid repeated freeze-thaw cycles. | |||||||||
| Name | Monkeypox virus (MPXV) antigen | ||||||
| Catalog Numbers | C1618 | C1619 | C1620 | C1621 | C1622 | C1624 | C1625 |
| Gene Name | A29L | A35R | B6R | M1R | |||
| Species | Monkeypox Virus | ||||||
| Type | Recombinant | ||||||
| Tag | His | ||||||
| Expression System | Eukaryotic | Prokaryotic | Eukaryotic | Prokaryotic | Eukaryotic | Eukaryotic | Prokaryotic |
| Uniprot ID | Q9YN60 | Q80KX2 | Q773E2 | Q80KX3 | |||
| Predicted MW | 13.3 kDa (including tag) | 14.3 kDa (including tag) | 31.6 kDa (including tag) | 20.4 kDa (including tag) | |||
| SDS-PAGE |
The predicted molecular weights of A29L, A35R, B6R, M1R are 13.3 kDa, 14.3 kDa, 31.6 kDa, 20.4 kDa respectively. |
||||||
| Purity | >90%, assessed by R250-stained SDS-PAGE | ||||||
| Buffer | 1x PBS, pH 7.4 | ||||||
| Storage | Store at -80°C, aliquot to avoid repeated freeze-thaw cycles | ||||||
Using ELISA, the Monkeypox virus A35R antigen (C1620, C1621) was used to detect MPXV A35R monoclonal antibodies R383e5, R387c6, R386t7, R384c2, and R392a3. The EC50 values for R383e5 and R387c6 were 7.29 ng/mL and 9.86 ng/mL, respectively.
Figure 1: ELISA results for A35R antibody (R383e5)
Figure 2: ELISA results for A35R antibody (R387c6)
Using ELISA, the Monkeypox virus A29L antigen (C1619) was used to detect MPXV A29L monoclonal antibody R395a6. The EC50 was 10.54 ng/mL.
Figure 3: ELISA results for A29L antibody (R395a6)
Using ELISA, the Monkeypox virus B6R antigen (C1622) was used to detect MPXV B6R monoclonal antibody R401c8. The EC50 was 15.26 ng/mL.
Figure 4: ELISA results for B6R antibody (R401c8)
Colloidal gold strips were prepared using the Monkeypox virus A35R antibody. The strips were coated with A35R antibodies R386t7, R387c6, and R384c2, and labeled with A35R antibody R392a3. The A35R antigen (C1620) was detected at concentrations of 0, 0.01, 0.1, 1, 10, and 100 ng/mL. The T line color showed a clear gradient change, with all three antibody pairs having a detection sensitivity of 10 pg/mL.
Figure 5: Colloidal gold detection results for Monkeypox virus A35R antibody
Colloidal gold strips were prepared using the Monkeypox virus A29L antibody. The strips were coated with A29L antibody R396t5 and labeled with A29L antibodies R399e2 and R397e8. The A29L antigen (C1619) was detected at concentrations of 0, 0.01, 0.1, 1, 10, and 100 ng/mL. The T line color showed a clear gradient change, with both antibody pairs having a detection sensitivity of 10 pg/mL.
Figure 6: Colloidal gold detection results for Monkeypox virus A29L antibody
Colloidal gold strips were prepared using the Monkeypox virus B6R antibody. The strips were coated with B6R antibody R401c8 and labeled with B6R antibody R400e7. The B6R antigen (C1622) was detected at concentrations of 0, 0.01, 0.1, 1, 10, and 100 ng/mL. The T line color showed a clear gradient change, with a detection sensitivity of 10 pg/mL for both antibodies.
Figure 7: Colloidal gold detection results for Monkeypox virus B6R antibody
Colloidal gold strips were prepared using the Monkeypox virus M1R antibody. The strips were coated with M1R antibody R417t6 and labeled with M1R antibody R418t3. The M1R antigen (C1624) was detected at concentrations of 0, 0.0125, 0.05, 0.1, and 1 ng/mL. The T line color showed a clear gradient change, with a detection sensitivity of 10 pg/mL for both antibodies.
Figure 8: Colloidal gold detection results for Monkeypox virus M1R antibody
Reference
1.Sarah Keasey, Christine Pugh, Alexander Tikhonov, et al. "Proteomic Basis of the Antibody Response to Monkeypox Virus Infection Examined in Cynomolgus Macaques and a Comparison to Human Smallpox Vaccination." Plos One, 2010, 5(12): e15547.
2.Emmanuel Alakunle, Ugo Moens, Godwin Nchind, et al. "Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution." Viruses, 2020, 12(11): 1257.
3.S. N. Shchelkunov, A. V. Totmenin, P. F. Safronov, et al. "Analysis of the Monkeypox Virus Genome." Virology, 2002, 297: 172-194.
